Jui-Chuang Wu, R&D Center for Membrane Technology and Chemical Engineering Department, Chung Yuan Christian University, 200 Chung Pei Rd., Chung Li, Tao Yuan, 32023, Taiwan and Huang-Chin Yang, Chemical Engineering Department, Chung Yuan Christian University, 200 Chung Pei Rd., Chung Li, Tao Yuan, 32023, Taiwan.
The later-flow immunochromatography is one of modern rapid detection approaches using the principle of specific immunity between antigens and affinity antibodies. Instead of antigen-antibody immunoassay, this study employed the specificity between DNA-ligand and anti-ligand antibody to detect Avian Influenza H5N2, in a rapid and convenient manner. The single-stranded target DNA amplified by Polymerase Chain Reaction (PCR) and labeled with a specific ligand was loaded onto nitrocellulose membrane to laterally flow through and captured by an anti-ligand antibody. The detection result was designed to be visually read out by gold-sol and identified Avian Influenza H5N2.
Keywords: lateral-flow immunochromatograph, DNA detection, Avian Influenza, nitrocellulose membrane, immunoassay