Neuroblastoma (NB) is the most common solid tumor of children, derived from the sympathetic nervous system. Since intensive therapeutic intervention does not prolong the overall disease-free survival rate for this tumor, novel therapeutic strategies are required. It has been reported that disialoganglioside (GD2) is abundantly expressed on the surface of NB cells and minimally in normal tissues. Hence, GD2 is an ideal targeting ligand for NB cells. On the other hand, delivery of carbon nanotubes (CNTs) into mammalian cells via endocytic pathway has recently gained increasing interests because of its potential therapeutic applications. In this study, we examined the specific binding of anti-GD2-tagged multi-walled CNTs against GD2-positive NB cell lines in vitro and the ensuing ingestion of fluorescent labeled anti-GD2-CNTs were investigated. Since optically stimulated electronic excitation of CNTs could be transferred to molecular vibration energy and causing heating, the study of increasing necrosis of malignant NB cells specifically ingested anti-GD2-CNTs is worth of exploring as a promising treatment. Our results showed that after CNTs were internalized via targeting onto cell surface, washed and irradiated with near-infrared (808 nm) laser for 15 min under intensity of 6 W/cm2, large fraction of GD2-expressing NB cells were non-viable as detected by calcein AM dye. It is expected that site-targeting of NB cells with anti-GD2 conjugated CNTs and further ablation of CNT-laden malignant cells via near-infrared laser induced over-physiological temperature is an alternative NB therapy.