586h Improving Bacteria Separation Using Green Chemistry

Audrey Buttice1, Kathryn Bailey2, Joyce Stroot3, Daniel Lim3, Peter Stroot2, and Norma Alcantar1. (1) Chemical and Biomedical Engineering, University of South Florida, 4202 East Fowler Ave. ENB 118, Tampa, FL 33620, (2) Civil and Environmental Engineering, University of South Florida, 4202 East Fowler Ave. ENB 118, Tampa, FL 33620, (3) Biology-Advanced Biosensors Laboratory, University of South Florida, 4202 East Fowler Ave. ENB 118, Tampa, FL 33620

As more and more attention is drawn to solving water pollution problems, particularly in developing countries where unsanitary storage is an issue, needs to develop inexpensive, effective and sustainable methods of pathogen removal are increasing. We have been studying an alternative to the modern technology that relief attempts are currently implanting in troubled areas. Through simple separation techniques a natural compound, known as cactus mucilage, can be extracted from the Opuntia ficus-indica cactus that is commonly found throughout the world. Experiments performed with sediments and heavy metals have suggested that this cactus mucilage is an effective tool for clearing contaminants from drinking water. Our experiments involve the removal of Escherichia coli and Bacillus cereus bacteria suspended in synthetic hard and soft waters. Methods include the use of column tests and plate counts to determine the speed and efficiency of bacteria removal. With the aid of CaCl2, settling has been observed in as little as five to ten minutes and removal rates of up to 97% have been achieved. Concentrations of mucilage and CaCl2 as well as pH, have all proven to play an important role in the effectiveness of the removal and optimal conditions have been determined. Cactus mucilage is an ideal material for water treatment because it is a natural substance, is of low cost, simple to use and is readily obtained and processed. The use of this type of green chemistry not only shows promise as a resource for achieving potable drinking water, but it also overcomes many of the problems witnessed with current relief projects. Its characteristics of use without maintenance and concerns regarding environmental impacts, grant it the possibility of reaching a large range of low-income communities.