Cells respond against adverse conditions by expressing a set of proteins, some of which are historically referred to as Heat Shock Proteins. While the name originated from a cell's expression response to a sudden increase in temperature, from a physiological point of view the expression of these proteins are a defense mechanism and is associated with the synthesis of proteins since they act as chaperons.

In this work, we have evaluated the expression of stress proteins under different hydrodynamic conditions and we analyzed the relationship between the expression of stress proteins and culture performance. Also, the expression of stress proteins in highly sensitive cell lines will be discussed. Stress proteins investigated have been studied in the context of heat and nutritional stress but it has not been determined the expression of stress proteins in response to hydrodynamic conditions.

The results strongly suggest that the expression of stress proteins follows a characteristic profile that is related to the state of the culture.