Songtao Wu1, Xiaodong Tong1, Archana Trivedi2, Hongfei Jia3, Minjuan Zhang3, Masahiko Ishii4, and Ping Wang5. (1) Bioproducts and Biosystems Engineering, University of Minnesota, 1475 Gortner Ave., 322 Snyder Hall, St. Paul, MN 55108, (2) Chemical and Biomolecular Engineering, University of Akron, 200 E. Buchtel Commons, Whitby 301, Akron, OH 44325, (3) Materials Research, Toyota Motor Engineering and Manufacturing North America, Inc., Ann Arbor, MI 48105, (4) Material Engineering Div. I, Toyota Motor Corporation, 1, Toyota-cho, Toyota, Aichi, 471-8572, Japan, (5) Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108
Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for reparation of bioactive materials through a spin coating process. Specifically,we investigated the preparation of protease Subtilisin Carlsberg-coated plastic films and examined their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic coatings with a typical loading of 13 ug/cm2, retaining 46% of the enzyme activity for hydrolysis of CEA in aqueous solutions. Interestingly, the surface-coated protease thin film not only catalyzed the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid-state CEA that was coated on top of the enzyme thin film.
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www.bbe.umn.edu/faculty/pingwang/