We have optimized an engineered version of the type III secretion system of Salmonella for the expression and secretion of enzymes that break down cellulose, known as cellulases. Previously we have shown that this system could be used to express and secrete spider silk monomers without adversely affecting cell growth or viability. Building on that effort, we now have developed and experimentally verified a model of the regulatory dynamics of the system, and applied the information that we learned from this model to increase the time of expression for cellulases or other genes of interest. A number of potential applications for our synthetic cellulase secretion system, including the high-throughput screening of cellulases and sets of cellulases for improved activity, will be discussed.