Anubhav Tripathi1, Gloria Oliver2, Chritina Simpson1, Matthew B. Kerby1, and Anuj Chauhan3. (1) Engineering, Brown University, Box D, Providence, RI 02912, (2) Chemical Engineering, Johns Hopkins University, Baltimore, MD 21218, (3) Chemical Engineering, University of Florida, Bldg 723, Room 411, Department of Chemical Engineering, University of Florida, Gainesville, FL 32611
We present a systematic study of the electrophoretic migration of 10 to 200 kDa protein fragments in dilute-polymer solutions using microfluidic chips. The electrophoretic mobility and dispersion of protein samples were measured in a series of monodisperse polydimethylacrylamide (PDMA) polymers of different molecular weights (243, 443 & 764 kDa, polydispersivity index < 2) of varying concentration. The polymer solutions were characterized using rheometry. Prior to loading onto the microchip, the polymer solution was mixed with known concentrations of sodium dodecylsulfate (SDS) surfactant and a staining dye. SDS-denatured protein samples were electrokinetically injected, separated and detected in the microchip using electric fields ranging from 100 to 300 V/cm.
Our results show that the electrophoretic mobility of protein fragments decreases exponentially with the concentration c of the polymer solution. The mobility was found to decrease logarithmically with the molecular weight of the protein fragment. In addition, the mobility was found to be independent of the electric field in the separation channel. The dispersion is relatively independent of polymer concentration and it first increases with protein size and then decreases with a maximum at about 45 kDa. The resolution power of the device decreases with concentration of PDMA solution but it is always better than the 10% of protein size. The protein migration does not seem to correspond to the Ogston or the reptation models. A semi-empirical expression for mobility given by van Winkle fits the data very well.