The monolayer coating of amphiphilic compounds onto surfaces is commonly achieved via the Langmuir-Blodgett (LB) technique. Such a technique exploits the hydrophobic and hydrophilic properties of amphiphiles to self-orient into a monolayer at the air-water interface with the hydrophilic head groups associated with water and the hydrophobic tails directed into the air. While LB monolayers of fatty acids or phospholipids have been well established, little is known about LB films of quantum dots (Qdots). This study successfully demonstrated that stable cadmium selenide (CdSe) Qdot Langmuir monolayers were formed at the air-water interface. The effect of Qdot capping ligands [tri-n-octylphosphine oxide (TOPO), dodecanethiol (DDT), and dithiocarbamates (DTC)] on the stability of the Langmuir monolayer was investigated in detail, and a theoretical model was used to describe Qdot desorption. Pressure-controlled experiments indicated that TOPO-capped Qdots formed the most stable Langmuir monolayers. The effect of deposition pressure on the transfer of DDT-capped CdSe Qdots onto a smooth silicon (100) substrate was correlated with the observed density of Qdots via atomic force microscopy (AFM). Finally, DDT-capped Qdots (3.4 nm) were LB deposited on an azide-terminated, self-assembled monolayer substrate, which was subsequently illuminated. Partial photocatalytic reduction of azides to amines by deposited CdSe Qdots was confirmed by XPS. Controlled deposition of Qdots followed by photocatalytic reduction of azide to amine under light irradiation can potentially be used to synthesize chemical patterns on surfaces with a tight pitch.