Monitoring and Control of Recombinant Protein Production - from Up-stream to Integrated Down-stream Processes
| Author: | Luttmann Reiner, Hamburg University of Applied Sciences, Germany |
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| Topic: | 8.4 Control of Biotechnological Processes |
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| Session: | From Drug Design to Production: Control Issues |
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| Keywords: | Recombinant proteins, API, E. coli, Pichia pastoris,global bioreactor monitoring, adaptive oxygen feed control,control of cell specific reaction rates, Integrated Bioprocessing,cross flow filtration, modeling of protein expression |
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Abstract
The production of intra cellular heterologous proteins withEscherichia coli has a demand of a global monitoring andcontrol system for optimization of complex and uncertain processbehavior.Moreover the expression of APIs - active pharmaceuticalingredients and its secretion by the yeast Pichia pastorisenables the development of Integrated Bioprocesses with directproduct harvest via cross flow filtration methods.This paper describes some associated monitoring and controlproblems.
